Myzus persicae insecticide resistance pdf

Guidelines for preventing and managing insecticide resistance in a phids on potatoes updated february 2014 these guidelines apply primarily to the potato aphidpeach myzus persicae in potato crops as this is the aphid species with the most significant insecticide resistance issues associated with it. In the present study, the fitness and energy costs that might be experienced by m. It is also acts as a vector for the transport of plant viruses such as cucumber mosaic virus cmv, potato. Mace pirimicarb kdr and superkdr pyrethroids 0 20 40 60 80 100 % samples containing resistant aphids mace myzus persicae field samples containing mace,kdrandsuperkdr. A common resistance mechanism against 2 of the most widely used insecticide classes, organophosphates and. We have now identified two forms of targetsite resistance involving. Characterisation of insecticide resistance mechanisms in italian populations of the green peach aphid myzus persicae sulzer coordinator. Anstead ja, williamson ms, denholm i 2008 new methods for the detection of insecticide resistant myzus persicae in u. Major mechanisms of insecticide resistance in green peach. Genetic diversity and insecticide resistance of myzus persicae hemiptera. Research transcriptomic responses of the aphid myzus. Pdf the evolution of insecticide resistant in the peach.

Guidelines for preventing and managing insecticide. We have now identified two forms of targetsite resistance involving changes in the acetylcholinesterase ache. Insecticide resistance and its implications for potato production in the uk ref. Aphids are a group of insects that have become global pests in agriculture and frequently exhibit insecticide.

Pesticide resistance guidelines for preventing and. The green peach aphid has a welldocumented history of insecticide resistance to most classes of insecticides foster et al. Quantification of insecticide resistance in the tobaccoadapted form of the green peach aphid, myzus persicae sulzer hemiptera. Green peach aphid myzus persicae sulzer is a cosmopolitan and polyphagous pest. Gene amplification in myzus persicae permits the synthesis of large quantities of esterase that cause resistance by detoxifying a wide range of insecticides. Advances in understanding insecticide resistance in the peach. After a brief introduction, the biochemistry of the resistance mechanism is discussed, followed by a description of how the understanding of this has been used to develop sensitive and reliable methods for monitoring resistance in. Williamson1, chris bass1 1centre for sustainable pest and disease management, rothamsted research, harpenden, united. The peach potato aphid, myzus persicae is a globally distributed crop pest with a host range of over 400 species including many economically important crop plants. The guidelines apply primarily to the peachpotato aphid myzus persicae on potato as this is the aphid species with the most. Uptodate information on insecticide resistance in m.

Silva, georg jander, horacio samaniego, john s ramsey, christian c. Amplification of a cytochrome p450 gene is associated with. Pdf the peachpotato aphid myzus persicae sulzer can resist a wide range of insecticides, but until recently 1990 the only mechanism. Insecticide resistance and its implications for potato. Aphids are a group of insects that have become global pests in agriculture and frequently exhibit insecticide resistance. The evolution of insecticide resistance in the peachpotato. Evidence that the e4 and fe4 esterase genes responsible for insecticide resistance in the aphid myzus persicae are part of a gene family. Insecticide treatment of the aphid myzus persicae sulzer has led to the evolution of several insecticide resistance mechanisms, including the detoxification of insecticides by elevated esterases. For its control, insecticide applications are widely used, but resistance to numerous molecules.

Monitoring insecticide resistance and diagnostics of. Studies on pirimicarb resistance mechanisms in iranian. To further characterize this resistance, we analyzed genomewide transcriptional. Ddt in that order, while the resistance levels were, in descending order, parathion, dichlorvos, diazinon, diazoxon, p, p. Pdf monitoring insecticide resistance and diagnostics of. Ten insecticides were assayed for toxicity against an unselected and a resistant strain of myzus persicae sulz.

Pdf insecticide resistance mechanisms in the green peach. I r m survey for resistance to four insecticides in myzus. Insecticide resistance by gene amplification in myzus persicae. A transcriptomic survey plos one, dec 2019 andrea x. Myzus persicae all states 315 625 g 14 days harvest grazing apply when aphids first appear using 30 100 l waterha ground rig or 20 50 lha by air. It is also acts as a vector for the transport of plant viruses such as cucumber mosaic virus cmv, potato virus y pvy and tobacco etch virus. How each resistance mechanism alone is likely to affect control of myzus persicae xxx good control, xx fair control, x poor control, none no ontrol likely. Myzus persicae, known as the green peach aphid or the peachpotato aphid, is a small green aphid. The study investigates whether genotypes that have an irm also have enhanced esterase. Guidelines for preventing and managing insecticide resistance. Strains resistant to demetonsmethyl and dimethoate in australia j.

Insecticide resistance mechanisms in the green peach. Norms of reaction in reproductive fitness rm of myzus persicae. The sensitive genotype exhibiting no resistance mechanism responded to the insecticide by upregulating 183 genes. Insecticide resistance mechanisms in the green peach aphid myzus persicae. A transcriptomic survey article pdf available in plos one 76.

This results from amplification of one of two closely related esterase genes e4 or fe4 with up to 80 copies in the most resistant aphids. The intensive use of insecticides to control this species has. These aphids also can be transported long distances by wind and storms. Response of myzus persicae sulzer to imidacloprid in potato varieties 264 based on the above grounds the present research project aimed to test two insecticides at labeled and reduced doses against m. High levels of resistance to carbamate and pyrethroid chemicals widespread in australian myzus persicae hemiptera. Evaluating reproductive fitness and metabolic costs for. Insecticide bioassays were carried out using topical application on three different populations collected from karaj and rasht in the northern provinces.

Myzus persicae sulzer is one of the most serious agricultural pests in china, and management strategies mainly rely on insecticidal treatment. Pdf myzus persicae sulzer is one of the most serious agricultural pests in china, and management strategies mainly rely on insecticidal treatment find. Intensification of insecticide resistance in uk field. After 40 generations, the azaselected line had developed 9fold resistance to aza compared to a nonselected control line, whereas the nseselected line did not. Temporal and spatial dynamics of insecticide resistance in. The peachpotato aphid myzus persicae sulzer can resist a wide range of insecticides, but until recently 1990 the only mechanism identified was the increased production of carboxylesterases e4 or fe4, which cause enhanced degradation and sequestration of insecticidal esters. The aphid myzus persicae develops insensitivity to almost all classes of insecticides through target site mutations. Of the six topically applied insecticides parathion was the most effective, followed by diazoxon, diazinon, aldrin, dichlorvos and p, p. Selection for resistance to azadirachtin in the green.

Two, slightly different forms of esterase can be elevated and the corresponding amplified genes show much homology, but differ at the 3. Amplification of a cytochrome p450 gene is associated with resistance to neonicotinoid insecticides in the aphid myzus persicae alin m. To further characterize this resistance, we analyzed genomewide transcriptional responses in three genotypes of m. Insecticide resistance in the aphid myzus persicae sulzer. To evaluate the resistance of field populations of m. Evidence for multiple origins of identical insecticides resistance mutations in the aphid myzus persicae. The green peach aphid, myzus persicae, has developed resistance to at least seventy different synthetic compounds, and different insecticide resistance mechanisms have been reported worldwide. Mechanisms of resistance to pirimicarb were surveyed in iranian populations of the peachpotato aphid, myzus persicae. We have now identified two forms of targetsite resistance involving changes in the. Challenges with managing insecticide resistance in uk pests. Pesticides and insecticides are crucial for agricultural productivity, global food security, and control of disease vectors. Insecticide resistance mechanisms in the green peach aphid myzus persicae hemiptera. A common resistance mechanism against 2 of the most widely used insecticide.

Wingless female adult green peach aphid, myzus persicae sulzer, with immatures. Background insecticide resistance is one of the best examples of rapid microevolution found in nature. Insecticide resistance in myzus persicae in australia j. The aim of this study was to assess the temporal and spatial distribution of resistant aphids that grow on peach trees and weeds, and establish the role of the secondary hosts as a reservoir of resistant genotypes. Neuere untersuchungen uber resistenzerscheinungen bei grunen pfirsichblattlaus myzus persicae sulzer gegenuber bisher gebrauchlechen insektiziden mitt. When young plants are infested in the greenhouse and then transplanted into the field, fields will not only be inoculated with aphids but insecticide resistance may be introduced. The evolution of insecticide resistance in the peach. Insecticide resistance warning for insecticide resistance management. The peach potato aphid, myzus persicae is the most economically important aphid pest worldwide. Challenges with managing insecticide resistance in uk. However, resistance to insecticides is a widespread and urgent problem, which leads to increased insecticide usage with dire consequences to the environment. How each resistance mechanism alone is likely to affect control of myzus persicae xxx good control, xx fair control, x poor control, none no ontrol likely it is important to remember that the scores in the above table are for each mechanism in isolation and can be cropdependent. This target site resistance mechanism was found in m.

Moores gd, devine gj, devonshire al 1994 insecticide insensitive acetylcholinesterase can enhance esterasebased resistance in myzus persicae and myzus nicotianae. Increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase e4 or its closely related variant, fe4, the degree of. Genetic diversity and insecticide resistance of myzus. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine. Gene amplification in myzus persicaepermits the synthesis of large quantities of esterase that cause resistance by detoxifying a wide range of insecticides. Selection for resistance to azadirachtin in the green peach. Chromosomal rearrangement involved in insecticide resistance of myzus persicae blackman, r.

The aphid myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. Life cycle and description the life cycle varies considerably, depending on the pres. Oct 29, 1998 the peachpotato aphid myzus persicae and the tobacco aphid myzus nicotianae have the same esterasebased mechanisms of insecticide resistance. Insecticide resistance mechanisms in the green peach aphid. Its mechanisms include mutations of target proteins which decreases their affinity for binding insecticide molecules as well as the enhanced production of metabolic enzymes, which detoxify andor sequester insecticides fenton et al. Populations of the peachpotato aphid myzus persicae sulzer heterozygous for a particular translocation between autosomes 1 and 3 were first discovered in glasshouses in great britain 1. Arthropod pesticide resistance database michigan state. In this work we i evaluated whether insecticide resistance mutations irm in m.

Their impact on their impact on the efficacy of insecticides used against m. Pdf the evolution of insecticide resistance in the peachpotato. The effect of aphid immigration on the rate of selection of insecticide resistance in myzus persicae by different classes of insecticides. Quantification of insecticide resistance in the tobacco.

The tobacco aphid myzus persicae nicotianae blackman is a subspecies of the highly polyphagous and agricultural pest myzus persicae sulzer. Molecular evidence that insecticide resistance in peach. Although resistance to insecticides provides important benefits for pests in agricultural fields that are treated with insecticides, it may be associated with fitness or. Studies of insecticide resistance in myzus persicae sulz. Amplification of a cytochrome p450 gene is associated with resistance to neonicotinoid insecticides in the aphid myzus persicae. Research transcriptomic responses of the aphid myzus persicae. Overcoming insecticide resistance through computational. To date, the neonicotinoids have remained an effective control measure however recent resistance monitoring work in southern europe represent a threat to the longterm efficacy of this chemical class. Aphididae lakshmipathi srigiriraju abstract the tobaccoadapted form of the green peach aphid, myzus persicae sulzer, is one of the most important insect pests of tobacco in the united states and around the world. The intensive use of insecticides to control this species over many years has led to populations that are now resistant to several classes of insecticide.

Insecticide resistance in the aphid myzus persicae. In both cases the focus was on discovering the mechanisms responsible for the neonicotinoid resistance, comparing patterns of gene expression between susceptible and resistant aphid. Quantification of insecticide resistance in the tobaccoadapted form of the green peach aphid, myzus persicae sulzerhemiptera. Analysis of insecticide resistant myzus persicae sulzer populations collected in italian peach orchards pest managment science, 58 975980. Two lines ofmyzus persicae of the same origin were treated repeatedlywith pure azadirachtin aza, or a refined neem seed extract nse, at the equivalent concentration of aza.

It is the most significant aphid pest of peach trees, causing decreased growth, shrivelling of the leaves and the death of various tissues. The evolution of insecticide resistance in the peach potato. Managing insecticide resistance in uk pests steve foster rothamstedresearch. Recently, two studies have targeted the identification of insecticide resistance mechanisms in m. Oct 15, 2019 pesticides and insecticides are crucial for agricultural productivity, global food security, and control of disease vectors. The wellestablished carboxylesterasebased resistance to insecticides in myzus persicae sulzer has recently been accentuated by the appearance of aphids with a modified acetylcholinesterase mace insensitive to pirimicarb and the novel aphicide, triazamate. Effect of decline of insecticide residues on selection for. Insecticide resistance has arisen independently in different green peach aphid populations on a number of occasions.

1605 858 491 1425 1382 759 896 1546 1413 225 488 81 707 1646 747 554 862 1566 688 764 1426 865 889 1382 1263 137 1524 204 967 354 1165 912 604 839 47 1395 1165 1311 590 890 950 256 1473 401 983 192